Characterization of a developmentally regulated amino acid transporter (AAT1p) of the rust fungus Uromyces fabae.
نویسندگان
چکیده
summary In the rust fungus Uromyces fabae, invasion of the host plant and haustorium formation are accompanied by the activation of many genes (PIGs =in planta induced genes). In addition to the previously described AAT2 (PIG2), AAT1 (PIG27) was found to encode a protein with a high similarity to fungal amino acid permeases. AAT1 transcripts are present in germinated hyphae and throughout the mycelium later in the infection process, but occur at the highest levels in haustoria. Expression of AAT1p in a histidine uptake-defective yeast mutant revealed energy-dependent transport of (14)C-histidine, with a K(M) value of 25.8 microm. In addition, complementation analysis revealed AAT1-dependent transport for lysine. Using Xenopus oocytes as expression system, AAT1p-dependent symport of protons with a broad spectrum of amino acids was observed, with the highest activities obtained with histidine and lysine. These results confirm that in rust fungi, the expression of amino acid transporters is developmentally regulated and occurs preferentially in the parasitic phase of development.
منابع مشابه
A putative amino acid transporter is specifically expressed in haustoria of the rust fungus Uromyces fabae.
A cDNA library constructed from haustoria of the rust fungus Uromyces fabae was screened for clones that are differentially expressed in haustoria. One family of cDNAs (in planta-induced gene 2 [PIG2] was isolated and found to encode a protein with high homologies to fungal amino acid transporters. A cDNA clone containing the complete coding region of PIG2 and the corresponding genomic clone we...
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عنوان ژورنال:
- Molecular plant pathology
دوره 3 1 شماره
صفحات -
تاریخ انتشار 2002